Gold in PDB 3p66: Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
Enzymatic activity of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
All present enzymatic activity of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme:
3.2.1.17;
Protein crystallography data
The structure of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme, PDB code: 3p66
was solved by
H.Wei,
Z.Wang,
J.Zhang,
S.House,
Y.-G.Gao,
L.Yang,
H.Robinson,
L.H.Tan,
H.Xing,
C.Hou,
I.M.Robertson,
J.-M.Zuo,
Y.Lu,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
10.00 /
1.36
|
Space group
|
P 43 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
78.543,
78.543,
37.154,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
21.9 /
24.3
|
Gold Binding Sites:
The binding sites of Gold atom in the Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
(pdb code 3p66). This binding sites where shown within
5.0 Angstroms radius around Gold atom.
In total 8 binding sites of Gold where determined in the
Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme, PDB code: 3p66:
Jump to Gold binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Gold binding site 1 out
of 8 in 3p66
Go back to
Gold Binding Sites List in 3p66
Gold binding site 1 out
of 8 in the Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
Mono view
Stereo pair view
|
A full contact list of Gold with other atoms in the Au binding
site number 1 of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Au301
b:49.0
occ:1.00
|
O
|
A:HOH2006
|
3.1
|
12.0
|
1.0
|
O
|
A:HOH2034
|
3.1
|
18.8
|
1.0
|
O
|
A:HOH2088
|
3.1
|
28.4
|
1.0
|
N
|
A:THR69
|
3.3
|
13.8
|
1.0
|
O
|
A:HOH2058
|
3.4
|
27.8
|
1.0
|
O
|
A:THR69
|
3.4
|
13.3
|
1.0
|
N
|
A:ARG68
|
3.6
|
12.5
|
1.0
|
CA
|
A:GLY67
|
3.6
|
13.4
|
1.0
|
OD1
|
A:ASN65
|
3.6
|
14.4
|
1.0
|
C
|
A:GLY67
|
3.6
|
13.7
|
1.0
|
OG
|
A:SER72
|
3.6
|
15.6
|
1.0
|
N
|
A:GLY67
|
3.6
|
11.3
|
1.0
|
C
|
A:THR69
|
3.7
|
10.7
|
1.0
|
O
|
A:HOH2017
|
3.8
|
15.2
|
1.0
|
CA
|
A:THR69
|
3.9
|
14.5
|
1.0
|
CB
|
A:THR69
|
4.3
|
10.7
|
1.0
|
O
|
A:GLY67
|
4.3
|
15.2
|
1.0
|
OD1
|
A:ASP66
|
4.3
|
11.3
|
1.0
|
O
|
A:HOH2123
|
4.3
|
22.3
|
1.0
|
C
|
A:ARG68
|
4.4
|
15.4
|
1.0
|
CA
|
A:ARG68
|
4.5
|
14.4
|
1.0
|
N
|
A:PRO70
|
4.5
|
13.8
|
1.0
|
AU
|
A:AU3307
|
4.5
|
50.8
|
1.0
|
C
|
A:ASP66
|
4.8
|
9.7
|
1.0
|
CB
|
A:SER72
|
4.8
|
13.3
|
1.0
|
CG
|
A:ASN65
|
4.8
|
10.5
|
1.0
|
OG1
|
A:THR69
|
4.9
|
12.0
|
1.0
|
N
|
A:ASP66
|
4.9
|
8.8
|
1.0
|
O
|
A:HOH2021
|
4.9
|
17.1
|
0.5
|
O
|
A:HOH2153
|
4.9
|
40.7
|
1.0
|
|
Gold binding site 2 out
of 8 in 3p66
Go back to
Gold Binding Sites List in 3p66
Gold binding site 2 out
of 8 in the Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
Mono view
Stereo pair view
|
A full contact list of Gold with other atoms in the Au binding
site number 2 of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Au302
b:48.5
occ:1.00
|
OH
|
A:TYR23
|
3.0
|
12.5
|
1.0
|
CE2
|
A:TYR23
|
3.7
|
9.0
|
1.0
|
CZ
|
A:TYR23
|
3.7
|
10.8
|
1.0
|
CA
|
A:GLY104
|
4.3
|
12.3
|
1.0
|
O
|
A:ARG21
|
4.7
|
13.5
|
1.0
|
N
|
A:GLY104
|
4.9
|
12.3
|
1.0
|
CE1
|
A:TYR23
|
4.9
|
12.2
|
1.0
|
CD2
|
A:TYR23
|
4.9
|
10.8
|
1.0
|
|
Gold binding site 3 out
of 8 in 3p66
Go back to
Gold Binding Sites List in 3p66
Gold binding site 3 out
of 8 in the Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
Mono view
Stereo pair view
|
A full contact list of Gold with other atoms in the Au binding
site number 3 of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Au303
b:57.1
occ:1.00
|
OG
|
A:SER24
|
3.1
|
17.0
|
1.0
|
N
|
A:GLY26
|
3.2
|
11.4
|
1.0
|
CA
|
A:GLY26
|
3.5
|
12.0
|
1.0
|
CB
|
A:SER24
|
3.5
|
15.6
|
1.0
|
CA
|
A:GLN121
|
3.6
|
14.7
|
1.0
|
CD1
|
A:ILE124
|
3.9
|
21.4
|
1.0
|
CB
|
A:GLN121
|
3.9
|
19.5
|
1.0
|
N
|
A:GLN121
|
4.0
|
15.2
|
1.0
|
CG
|
A:GLN121
|
4.1
|
27.7
|
1.0
|
CG1
|
A:ILE124
|
4.2
|
18.7
|
1.0
|
N
|
A:LEU25
|
4.3
|
13.8
|
1.0
|
C
|
A:LEU25
|
4.4
|
12.7
|
1.0
|
O
|
A:VAL120
|
4.4
|
13.0
|
1.0
|
C
|
A:VAL120
|
4.4
|
12.6
|
1.0
|
CG2
|
A:VAL120
|
4.4
|
15.2
|
1.0
|
C
|
A:SER24
|
4.4
|
12.2
|
1.0
|
C
|
A:GLY26
|
4.5
|
10.1
|
1.0
|
CA
|
A:SER24
|
4.6
|
12.6
|
1.0
|
N
|
A:ASN27
|
4.7
|
11.7
|
1.0
|
C
|
A:GLN121
|
4.8
|
14.7
|
1.0
|
CA
|
A:LEU25
|
4.8
|
12.1
|
1.0
|
O
|
A:SER24
|
4.9
|
12.0
|
1.0
|
|
Gold binding site 4 out
of 8 in 3p66
Go back to
Gold Binding Sites List in 3p66
Gold binding site 4 out
of 8 in the Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
Mono view
Stereo pair view
|
A full contact list of Gold with other atoms in the Au binding
site number 4 of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Au304
b:63.9
occ:1.00
|
ND2
|
A:ASN65
|
3.1
|
14.2
|
1.0
|
O
|
A:HOH2174
|
3.2
|
43.0
|
1.0
|
CA
|
A:GLY67
|
3.7
|
13.4
|
1.0
|
N
|
A:GLY67
|
4.1
|
11.3
|
1.0
|
CG
|
A:ASN65
|
4.2
|
10.5
|
1.0
|
OD1
|
A:ASN65
|
4.5
|
14.4
|
1.0
|
O
|
A:HOH2088
|
4.6
|
28.4
|
1.0
|
C
|
A:ASP66
|
4.7
|
9.7
|
1.0
|
O
|
A:HOH2102
|
4.8
|
39.9
|
1.0
|
O
|
A:ASP66
|
4.9
|
11.5
|
1.0
|
|
Gold binding site 5 out
of 8 in 3p66
Go back to
Gold Binding Sites List in 3p66
Gold binding site 5 out
of 8 in the Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
Mono view
Stereo pair view
|
A full contact list of Gold with other atoms in the Au binding
site number 5 of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Au305
b:55.6
occ:0.50
|
OG1
|
A:THR51
|
2.6
|
9.5
|
1.0
|
OD2
|
A:ASP66
|
2.8
|
11.0
|
1.0
|
NE
|
A:ARG68
|
2.8
|
20.9
|
1.0
|
O
|
A:GLY49
|
2.9
|
15.4
|
1.0
|
OG1
|
A:THR69
|
3.3
|
12.0
|
1.0
|
NE
|
A:ARG45
|
3.4
|
26.9
|
1.0
|
CB
|
A:THR51
|
3.4
|
7.9
|
1.0
|
CG
|
A:ARG68
|
3.4
|
14.0
|
1.0
|
N
|
A:THR51
|
3.4
|
9.0
|
1.0
|
C
|
A:SER50
|
3.6
|
9.7
|
1.0
|
CD
|
A:ARG68
|
3.6
|
15.3
|
1.0
|
CZ
|
A:ARG68
|
3.7
|
23.2
|
1.0
|
CG
|
A:ASP66
|
3.7
|
10.3
|
1.0
|
CZ
|
A:ARG45
|
3.7
|
23.9
|
1.0
|
NH2
|
A:ARG68
|
3.7
|
17.6
|
1.0
|
NH2
|
A:ARG45
|
3.8
|
14.1
|
1.0
|
CA
|
A:SER50
|
3.8
|
11.8
|
1.0
|
O
|
A:ARG68
|
3.8
|
17.4
|
1.0
|
OD1
|
A:ASP66
|
3.9
|
11.3
|
1.0
|
OG
|
A:SER60
|
4.0
|
10.0
|
1.0
|
C
|
A:GLY49
|
4.0
|
13.8
|
1.0
|
CA
|
A:THR51
|
4.1
|
10.2
|
1.0
|
CD
|
A:ARG45
|
4.1
|
15.1
|
1.0
|
O
|
A:SER50
|
4.2
|
10.8
|
1.0
|
OH
|
A:TYR53
|
4.3
|
10.1
|
1.0
|
C
|
A:ARG68
|
4.3
|
15.4
|
1.0
|
N
|
A:SER50
|
4.4
|
12.5
|
1.0
|
CB
|
A:ARG68
|
4.6
|
11.2
|
1.0
|
CB
|
A:THR69
|
4.6
|
10.7
|
1.0
|
CG2
|
A:THR51
|
4.6
|
10.5
|
1.0
|
NH1
|
A:ARG45
|
4.7
|
48.0
|
1.0
|
N
|
A:THR69
|
4.9
|
13.8
|
1.0
|
NH1
|
A:ARG68
|
4.9
|
19.0
|
1.0
|
CB
|
A:SER60
|
4.9
|
10.2
|
1.0
|
O
|
A:HOH2107
|
5.0
|
31.6
|
1.0
|
CB
|
A:ASP66
|
5.0
|
11.0
|
1.0
|
CA
|
A:ARG68
|
5.0
|
14.4
|
1.0
|
CA
|
A:THR69
|
5.0
|
14.5
|
1.0
|
|
Gold binding site 6 out
of 8 in 3p66
Go back to
Gold Binding Sites List in 3p66
Gold binding site 6 out
of 8 in the Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
Mono view
Stereo pair view
|
A full contact list of Gold with other atoms in the Au binding
site number 6 of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Au306
b:75.9
occ:1.00
|
O
|
A:HOH2104
|
3.0
|
32.3
|
1.0
|
O
|
A:HOH2060
|
3.0
|
19.6
|
1.0
|
ND2
|
A:ASN65
|
3.5
|
14.2
|
1.0
|
CB
|
A:PRO79
|
3.8
|
11.2
|
1.0
|
CA
|
A:PRO79
|
3.8
|
11.0
|
1.0
|
CB
|
A:ASN65
|
3.9
|
10.7
|
1.0
|
N
|
A:PRO79
|
3.9
|
11.2
|
1.0
|
CG
|
A:ASN65
|
4.2
|
10.5
|
1.0
|
CD
|
A:PRO79
|
4.3
|
11.8
|
1.0
|
C
|
A:ILE78
|
4.4
|
11.3
|
1.0
|
O
|
A:ASN65
|
4.5
|
10.7
|
1.0
|
CG
|
A:PRO79
|
4.6
|
15.0
|
1.0
|
O
|
A:HOH2102
|
4.6
|
39.9
|
1.0
|
O
|
A:ILE78
|
4.7
|
11.6
|
1.0
|
ND2
|
A:ASN74
|
4.7
|
14.5
|
1.0
|
|
Gold binding site 7 out
of 8 in 3p66
Go back to
Gold Binding Sites List in 3p66
Gold binding site 7 out
of 8 in the Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
Mono view
Stereo pair view
|
A full contact list of Gold with other atoms in the Au binding
site number 7 of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Au307
b:50.8
occ:1.00
|
O
|
A:SER60
|
2.3
|
12.2
|
1.0
|
O
|
A:HOH2006
|
2.3
|
12.0
|
1.0
|
O
|
A:CYS64
|
2.3
|
10.4
|
1.0
|
OG
|
A:SER72
|
2.4
|
15.6
|
1.0
|
O
|
A:HOH2017
|
2.4
|
15.2
|
1.0
|
O
|
A:ARG73
|
2.5
|
14.2
|
1.0
|
CB
|
A:SER72
|
3.2
|
13.3
|
1.0
|
C
|
A:SER60
|
3.4
|
12.5
|
1.0
|
C
|
A:CYS64
|
3.5
|
9.4
|
1.0
|
C
|
A:ARG73
|
3.5
|
15.2
|
1.0
|
CA
|
A:ASN65
|
3.9
|
10.1
|
1.0
|
CA
|
A:SER60
|
4.0
|
9.4
|
1.0
|
N
|
A:ARG73
|
4.0
|
16.6
|
1.0
|
C
|
A:SER72
|
4.2
|
19.0
|
1.0
|
N
|
A:ASN65
|
4.2
|
10.0
|
1.0
|
CB
|
A:SER60
|
4.2
|
10.2
|
1.0
|
CA
|
A:SER72
|
4.3
|
17.2
|
1.0
|
CA
|
A:ARG73
|
4.4
|
15.0
|
1.0
|
N
|
A:ASN74
|
4.4
|
13.1
|
1.0
|
N
|
A:CYS64
|
4.4
|
11.1
|
1.0
|
AU
|
A:AU3301
|
4.5
|
49.0
|
1.0
|
N
|
A:ARG61
|
4.5
|
12.3
|
1.0
|
OD1
|
A:ASN65
|
4.6
|
14.4
|
1.0
|
N
|
A:ASP66
|
4.6
|
8.8
|
1.0
|
C
|
A:ARG61
|
4.6
|
13.4
|
1.0
|
CB
|
A:THR69
|
4.6
|
10.7
|
1.0
|
CA
|
A:CYS64
|
4.6
|
9.7
|
1.0
|
O
|
A:SER72
|
4.6
|
17.1
|
1.0
|
O
|
A:ARG61
|
4.6
|
17.0
|
1.0
|
CA
|
A:ASN74
|
4.7
|
13.8
|
1.0
|
O
|
A:HOH2172
|
4.7
|
33.8
|
1.0
|
CB
|
A:ASN74
|
4.8
|
13.6
|
1.0
|
CB
|
A:ASN65
|
4.8
|
10.7
|
1.0
|
C
|
A:ASN65
|
4.8
|
7.3
|
1.0
|
CA
|
A:ARG61
|
4.9
|
13.5
|
1.0
|
N
|
A:TRP62
|
4.9
|
11.2
|
1.0
|
O
|
A:THR69
|
4.9
|
13.3
|
1.0
|
OG1
|
A:THR69
|
5.0
|
12.0
|
1.0
|
|
Gold binding site 8 out
of 8 in 3p66
Go back to
Gold Binding Sites List in 3p66
Gold binding site 8 out
of 8 in the Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme
Mono view
Stereo pair view
|
A full contact list of Gold with other atoms in the Au binding
site number 8 of Time-Dependent and Protein-Directed in Situ Growth of Gold Nanoparticles in A Single Crystal of Lysozyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Au308
b:59.3
occ:1.00
|
OG1
|
A:THR43
|
2.7
|
16.2
|
1.0
|
O
|
A:GLN41
|
2.8
|
13.2
|
1.0
|
N
|
A:THR43
|
3.1
|
11.2
|
1.0
|
CB
|
A:THR43
|
3.5
|
10.1
|
1.0
|
CG2
|
A:THR43
|
3.6
|
11.5
|
1.0
|
C
|
A:ALA42
|
3.8
|
9.9
|
1.0
|
CA
|
A:THR43
|
3.8
|
11.0
|
1.0
|
C
|
A:GLN41
|
3.9
|
13.8
|
1.0
|
CA
|
A:ALA42
|
4.0
|
9.8
|
1.0
|
O
|
A:THR43
|
4.3
|
15.7
|
1.0
|
N
|
A:ALA42
|
4.4
|
10.2
|
1.0
|
C
|
A:THR43
|
4.5
|
11.5
|
1.0
|
O
|
A:ALA42
|
4.7
|
8.5
|
1.0
|
|
Reference:
H.Wei,
Z.Wang,
J.Zhang,
S.House,
Y.G.Gao,
L.Yang,
H.Robinson,
L.H.Tan,
H.Xing,
C.Hou,
I.M.Robertson,
J.M.Zuo,
Y.Lu.
Time-Dependent, Protein-Directed Growth of Gold Nanoparticles Within A Single Crystal of Lysozyme. Nat Nanotechnol V. 6 93 2011.
ISSN: ISSN 1748-3387
PubMed: 21278750
DOI: 10.1038/NNANO.2010.280
Page generated: Wed Jul 10 14:19:12 2024
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