Gold in PDB 1w1d: Crystal Structure of the PDK1 Pleckstrin Homology (pH) Domain Bound to Inositol (1,3,4,5)-Tetrakisphosphate

All present enzymatic activity of Crystal Structure of the PDK1 Pleckstrin Homology (pH) Domain Bound to Inositol (1,3,4,5)-Tetrakisphosphate:
2.7.1.37;

The structure of Crystal Structure of the PDK1 Pleckstrin Homology (pH) Domain Bound to Inositol (1,3,4,5)-Tetrakisphosphate, PDB code: 1w1d was solved by D.Komander, M.Deak, D.R.Alessi, D.M.F.Van Aalten, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 1.5
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	35.399, 58.922, 36.578, 90.00, 101.48, 90.00
R / Rfree (%)	14.5 / 20

The binding sites of Gold atom in the Crystal Structure of the PDK1 Pleckstrin Homology (pH) Domain Bound to Inositol (1,3,4,5)-Tetrakisphosphate (pdb code 1w1d). This binding sites where shown within 5.0 Angstroms radius around Gold atom.
In total 2 binding sites of Gold where determined in the Crystal Structure of the PDK1 Pleckstrin Homology (pH) Domain Bound to Inositol (1,3,4,5)-Tetrakisphosphate, PDB code: 1w1d:
Jump to Gold binding site number: 1; 2;

Gold binding site 1 out of 2 in the Crystal Structure of the PDK1 Pleckstrin Homology (pH) Domain Bound to Inositol (1,3,4,5)-Tetrakisphosphate


Mono view


Stereo pair view

A full contact list of Gold with other atoms in the Au binding site number 1 of Crystal Structure of the PDK1 Pleckstrin Homology (pH) Domain Bound to Inositol (1,3,4,5)-Tetrakisphosphate within 5.0Å range: probe atom residue distance (Å) B Occ A:Au1551 b:18.1 occ:0.85 AU A:AU1551 0.0 18.1 0.8 SG A:CYS536 2.2 14.4 1.0 O A:HOH2025 2.7 24.4 1.0 AU A:AU1551 3.0 22.1 0.1 CB A:CYS536 3.2 11.2 1.0 C A:CYS536 3.2 10.1 1.0 O A:CYS536 3.3 10.9 1.0 N A:ARG537 3.5 10.5 1.0 CA A:CYS536 3.7 9.9 1.0 CA A:ARG537 3.9 11.0 1.0 CB A:GLN540 4.1 10.7 1.0 O A:HOH2182 4.1 32.9 1.0 CG A:PRO506 4.5 11.9 1.0 CG A:ARG537 4.6 18.1 1.0 CG A:GLN540 4.7 13.5 1.0 O A:HOH2149 4.8 27.5 1.0 CB A:ARG537 4.8 13.0 1.0 O A:PRO506 4.9 14.6 1.0 C A:ARG537 4.9 10.1 1.0

Gold binding site 2 out of 2 in the Crystal Structure of the PDK1 Pleckstrin Homology (pH) Domain Bound to Inositol (1,3,4,5)-Tetrakisphosphate


Mono view


Stereo pair view

A full contact list of Gold with other atoms in the Au binding site number 2 of Crystal Structure of the PDK1 Pleckstrin Homology (pH) Domain Bound to Inositol (1,3,4,5)-Tetrakisphosphate within 5.0Å range: probe atom residue distance (Å) B Occ A:Au1551 b:22.1 occ:0.15 AU A:AU1551 0.0 22.1 0.1 SG A:CYS536 2.4 14.4 1.0 O A:HOH2149 2.5 27.5 1.0 AU A:AU1551 3.0 18.1 0.8 CB A:CYS536 3.3 11.2 1.0 N A:ALA508 3.3 17.1 1.0 CB A:ALA508 3.6 18.6 1.0 CA A:ALA508 4.1 18.1 1.0 C A:GLU507 4.2 16.4 1.0 CA A:GLU507 4.4 15.9 1.0 O A:ALA508 4.6 20.6 1.0 CD2 A:HIS533 4.7 25.9 1.0 CA A:CYS536 4.7 9.9 1.0 C A:ALA508 4.9 18.1 1.0

D.Komander, A.Fairservice, M.Deak, G.S.Kular, A.R.Prescott, C.P.Downes, S.T.Safrany, D.R.Alessi, D.M.F.Van Aalten. Structural Insights Into the Regulation of PDK1 By Phosphoinositides and Inositol Phosphates Embo J. V. 23 3918 2004.
ISSN: ISSN 0261-4189
PubMed: 15457207
DOI: 10.1038/SJ.EMBOJ.7600379