Gold in PDB 1rif: Crystal Structure of the Uvsw Helicase From Bacteriophage T4

The structure of Crystal Structure of the Uvsw Helicase From Bacteriophage T4, PDB code: 1rif was solved by E.A.Sickmier, S.W.White, K.N.Kreuzer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	42.739, 95.181, 86.278, 90.00, 95.40, 90.00
R / Rfree (%)	21.8 / 24.6

The structure of Crystal Structure of the Uvsw Helicase From Bacteriophage T4 also contains other interesting chemical elements:

Magnesium

(Mg)

3 atoms

The binding sites of Gold atom in the Crystal Structure of the Uvsw Helicase From Bacteriophage T4 (pdb code 1rif). This binding sites where shown within 5.0 Angstroms radius around Gold atom.
In total 4 binding sites of Gold where determined in the Crystal Structure of the Uvsw Helicase From Bacteriophage T4, PDB code: 1rif:
Jump to Gold binding site number: 1; 2; 3; 4;

Gold binding site 1 out of 4 in the Crystal Structure of the Uvsw Helicase From Bacteriophage T4


Mono view


Stereo pair view

A full contact list of Gold with other atoms in the Au binding site number 1 of Crystal Structure of the Uvsw Helicase From Bacteriophage T4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Au287 b:29.8 occ:1.00 SG A:CYS234 2.5 22.2 1.0 CB A:CYS234 3.3 17.7 1.0 N A:HIS235 3.5 19.5 1.0 C A:CYS234 3.5 18.7 1.0 O A:CYS234 3.8 17.5 1.0 CA A:HIS235 3.9 20.7 1.0 CA A:CYS234 3.9 18.3 1.0 CD2 A:LEU262 4.0 25.4 1.0 CB A:SER259 4.1 21.3 1.0 CD2 A:TYR272 4.2 24.4 1.0 CB A:HIS235 4.2 18.2 1.0 CG2 A:ILE132 4.4 19.4 1.0 OG A:SER259 4.4 23.2 1.0 N A:CYS234 4.5 15.8 1.0 CD1 A:ILE132 4.5 18.0 1.0 CB A:TYR272 4.7 22.0 1.0 CG A:TYR272 4.8 24.0 1.0 CG1 A:ILE132 4.9 19.5 1.0 CE2 A:TYR272 5.0 25.1 1.0

Gold binding site 2 out of 4 in the Crystal Structure of the Uvsw Helicase From Bacteriophage T4


Mono view


Stereo pair view

A full contact list of Gold with other atoms in the Au binding site number 2 of Crystal Structure of the Uvsw Helicase From Bacteriophage T4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Au285 b:46.5 occ:1.00 SG A:CYS72 2.9 56.1 1.0 CB A:CYS72 3.1 45.5 1.0 CB A:ALA79 3.5 32.4 1.0 O A:ILE68 3.5 30.0 1.0 CG2 A:ILE68 3.8 30.6 1.0 CA A:LYS69 3.9 32.0 1.0 C A:ILE68 3.9 30.5 1.0 N A:LYS69 4.1 30.9 1.0 CD A:LYS69 4.2 38.1 1.0 O A:HOH386 4.4 38.3 1.0 CA A:CYS72 4.5 42.9 1.0 CB A:ILE68 4.5 30.0 1.0 O A:HOH333 4.5 35.7 1.0 N A:CYS72 4.7 41.2 1.0 CB A:LYS69 4.7 33.1 1.0 CA A:ALA79 4.7 32.5 1.0 N A:ALA79 4.8 34.0 1.0 C A:LYS69 4.9 31.6 1.0 CA A:ILE68 4.9 29.8 1.0 CD1 A:ILE81 5.0 33.0 1.0

Gold binding site 3 out of 4 in the Crystal Structure of the Uvsw Helicase From Bacteriophage T4


Mono view


Stereo pair view

A full contact list of Gold with other atoms in the Au binding site number 3 of Crystal Structure of the Uvsw Helicase From Bacteriophage T4 within 5.0Å range: probe atom residue distance (Å) B Occ B:Au283 b:37.9 occ:1.00 SG B:CYS234 2.5 22.0 1.0 O B:HOH384 3.3 36.2 1.0 N B:HIS235 3.5 20.5 1.0 C B:CYS234 3.6 20.2 1.0 CB B:CYS234 3.6 18.3 1.0 CA B:HIS235 3.8 21.1 1.0 O B:CYS234 4.0 20.7 1.0 CB B:SER259 4.0 22.2 1.0 CA B:CYS234 4.1 20.6 1.0 CB B:HIS235 4.1 22.0 1.0 CD1 B:TYR272 4.2 25.9 1.0 CG B:TYR272 4.3 26.2 1.0 CG2 B:ILE132 4.3 14.3 1.0 CE1 B:TYR272 4.4 28.3 1.0 OG B:SER259 4.5 21.5 1.0 CD2 B:TYR272 4.6 27.5 1.0 N B:CYS234 4.6 19.7 1.0 CZ B:TYR272 4.7 29.3 1.0 CB B:TYR272 4.8 24.0 1.0 CE2 B:TYR272 4.8 30.7 1.0 CD1 B:ILE132 4.8 14.7 1.0 CD1 B:LEU262 5.0 38.6 1.0 CG B:LEU262 5.0 37.8 1.0

Gold binding site 4 out of 4 in the Crystal Structure of the Uvsw Helicase From Bacteriophage T4


Mono view


Stereo pair view

A full contact list of Gold with other atoms in the Au binding site number 4 of Crystal Structure of the Uvsw Helicase From Bacteriophage T4 within 5.0Å range: probe atom residue distance (Å) B Occ B:Au284 b:39.3 occ:1.00 SG B:CYS72 2.6 30.2 1.0 O B:HOH351 3.0 24.1 1.0 CB B:CYS72 3.1 31.9 1.0 CB B:ALA79 3.5 26.9 1.0 O B:ILE68 3.5 26.5 1.0 CA B:LYS69 3.7 28.0 1.0 C B:ILE68 3.7 27.1 1.0 CG2 B:ILE68 3.7 29.7 1.0 N B:LYS69 3.8 27.3 1.0 CE B:LYS69 4.2 34.5 1.0 O B:HOH289 4.2 66.4 1.0 CB B:ILE68 4.3 27.9 1.0 CA B:CYS72 4.4 33.0 1.0 O B:HOH333 4.5 45.0 1.0 N B:CYS72 4.5 31.5 1.0 CD1 B:ILE81 4.6 30.3 1.0 C B:LYS69 4.6 28.1 1.0 CA B:ILE68 4.6 26.4 1.0 NZ B:LYS69 4.7 34.7 1.0 O B:LYS69 4.7 27.9 1.0 CB B:LYS69 4.7 28.2 1.0 CA B:ALA79 4.8 27.3 1.0 O B:HOH376 4.9 37.6 1.0 N B:ALA79 4.9 28.4 1.0 O B:HOH302 4.9 38.5 1.0

E.A.Sickmier, K.N.Kreuzer, S.W.White. The Crystal Structure of the Uvsw Helicase From Bacteriophage T4. Structure V. 12 583 2004.
ISSN: ISSN 0969-2126
PubMed: 15062081
DOI: 10.1016/J.STR.2004.02.016