Gold in the structure of Recognition of EIF4G By Rotavirus NSP3 Reveals A Basis For Mrna Circularization (pdb 1lj2)

The binding sites of Gold atom in the structure of Recognition of EIF4G By Rotavirus NSP3 Reveals A Basis For Mrna Circularization (pdb code 1lj2). This binding sites where shown with 5.0 Angstroms radius around Gold atom. The 1lj2 structure was solved by C.M.GROFT, S.K.BURLEY, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 20.0-2.4 Space group P212121 a (A) 59.690 b (A) 74.040 c (A) 77.460 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 22.1 Rfree (%) 27.2 Gold Binding Sites: Gold binding site 1 out of 3 in 1lj2 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Gold in the PDB 1lj2. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Gold atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Arg250, B: Lys259, B: Glu263, B: Hoh1228, conact list: Atom Atom Distance (A) Au CB B:Arg250 4.82 Au CD B:Arg250 4.68 Au CG B:Arg250 3.81 Au NE B:Arg250 4.61 Au CB B:Lys259 4.92 Au CD B:Lys259 4.58 Au OE2 B:Glu263 4.89 Au O B:Hoh1228 4.61 interactive model: Gold binding site 2 out of 3 in 1lj2 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Gold in the PDB 1lj2. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Gold atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Leu209, B: Leu209, B: Gln210, B: Ile213, B: Pro214, B: Hoh1223, conact list: Atom Atom Distance (A) Au CD1 A:Leu209 4.20 Au CD2 A:Leu209 4.51 Au CG A:Leu209 4.81 Au O B:Leu209 4.08 Au C B:Leu209 4.52 Au O B:Gln210 4.75 Au N B:Gln210 4.56 Au CB B:Gln210 4.95 Au C B:Gln210 4.82 Au CG B:Gln210 4.83 Au CA B:Gln210 4.09 Au CB B:Ile213 4.46 Au CD1 B:Ile213 4.07 Au CG2 B:Ile213 4.50 Au CG1 B:Ile213 4.87 Au CD B:Pro214 4.84 Au O B:Hoh1223 2.98 interactive model: Gold binding site 3 out of 3 in 1lj2 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 3 of Gold in the PDB 1lj2. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Gold atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: D: Pro145, D: Gly148, D: Gly149, D: Hoh1100, conact list: Atom Atom Distance (A) Au O D:Pro145 4.97 Au N D:Pro145 4.55 Au CB D:Pro145 3.85 Au CD D:Pro145 4.97 Au C D:Pro145 4.92 Au CA D:Pro145 3.83 Au O D:Gly148 4.68 Au C D:Gly148 4.60 Au N D:Gly149 4.05 Au C D:Gly149 4.89 Au CA D:Gly149 3.52 Au O D:Hoh1100 2.75 interactive model: